Table 1.
The theoretical thermodynamic parameters at TG for the thermal unfolding of pepsin at different pH values within the framework of this study.
| pH | TG (K) | ΔHcal (kcal mol−1) | ΔHDeffmodel (kcal mol−1) | ΔGDeffmodel (kcal mol−1) |
|---|---|---|---|---|
| The first transition | ||||
| 1.0 | — | — | — | — |
| 2.0 | 305.55±0.2 | 128±5 | 105.7 | 117.96 |
| 3.0 | 322.15±0.2 | 76±4 | 70.12 | –0.9 |
| 4.0 | 320.05±0.2 | 44±3 | 24.43 | 45.8 |
| The second transition | ||||
| 1.0 | 332.65±0.2 | 210±5 | 149.48 | 0.006 |
| 2.0 | 339.85±0.2 | 133±3 | 128.52 | –2.2 |
| 3.0 | 348.55±0.2 | 117±4 | 43.45 | –49.43 |
| 4.0 | 349.85±0.2 | 108±2 | 80.28 | –56.8 |