Fig 1. Effects of pharmacological inhibition of ENaC on vascular response mesenteric arteries.

(A) Vasoconstrictive response to phenylephrine (1.10⁻⁶ M), an α-adrenergic agonist. Response to phenylephrine was not modified after inhibition of ENaC. (B) Endothelium-dependent dilatory response induced by acetylcholine (1.10⁻⁶ M). Dilation of mesenteric arteries to Ach was decreased after benzamil preincubation. (C) Flow-mediated dilation in response to increased intraluminal shear stress. Dilation to flow was drastically decreased in vessels incubated with benzamil. Values are mean ± SEM (n = 3–10 for each group). *P<0.05, ***P<0.001.