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. 2016 Dec 8;9:121–127. doi: 10.1016/j.bbrep.2016.11.017

Fig. 2.

Fig. 2.

The experimental (•) and model fitted (—) pH profile of phosphate buffer prepared by mixing different volumes of equimolar concentrations of NaH2PO4 and Na2HPO4. (A) Shows the pH profile of 0.1 M phosphate buffer prepared by mixing mono and di sodium salt of phosphate. The experimental data was fitted to phosphate buffer model and optimized pKa values such as 2.239, 6.864, and 11.627 were obtained. (B) Shows the pH profile of 1 M phosphate buffer prepared by mixing mono and di sodium salt of phosphate. The experimental data was fitted to phosphate buffer model and optimized pKa values such as 2.15, 6.8537 and 8.768 were obtained.