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. 2016 Dec 8;9:121–127. doi: 10.1016/j.bbrep.2016.11.017

Table 1.

The pH profile of the 1 M phosphate buffer prepared by mixing different ratios of 1 M NaH2PO4 and 1 M Na2HPO4, The experimental values are model fitted using dynamic approach and the back-calculated pH values based on optimized pKa values are tabulated below.

Solution "A" (mL) Solution "B" (mL) 1 M Phosphate buffer (pH)
(1 M NaH2PO4) (1 M Na2HPO4) Experimental Back calculated
877 123 6.0 5.989
850 150 6.1 6.088
815 185 6.2 6.196
775 225 6.3 6.302
735 265 6.4 6.395
685 315 6.5 6.498
625 375 6.6 6.611
565 435 6.7 6.716
510 490 6.8 6.807
450 550 6.9 6.905
390 610 7.0 7.003
330 670 7.1 7.102
280 720 7.2 7.188