Fig. 3.

Effect of positional variation of FSS strength on ERK and AKT phosphorylation by orbital shaking. (A, upper panel) Schematic diagrams of “Whole” and “Peripheral” arrangements of the cells. (A, middle panel) MG-63 cells in whole or peripheral arrangements were exposed to orbital shaking, and then phosphorylation of ERK was measured by western blotting. (A, lower panel) Signal intensities of phosphorylated ERK were normalized to total ERK and plotted as ratios to unshaken (0 min) samples. Open bars, whole; closed bars, peripheral. Error bars, SD. *p<0.05; **p<0.01. (B) MG-63 cells were shaken at 120 and 160 rpm (upper panel) or 120 and 60 rpm for the indicated times, and then phosphorylation of ERK and AKT was measured by western blotting.