. 2017 Sep 26;12(9):e0185351. doi: 10.1371/journal.pone.0185351

Table 3. Invariant amino acids found within the highly conserved patterns in eukaryotes and comparing them with the established crystal structures.

Conserved blocks	Amino acid sequence ^a	MtMIPS ^a	TmMIPS ^a	AfMIPS ^a	ScMIPS ^a	CeMIPS ^a	OsMIPS ^a
Block A	`xnxcxDsll`	`klevwD₃₁₀sPN`	`grinD₃₁₆spa`	`iwdaiD₃₃₂aiv`	`hnvceD₄₃₈sll`	`hntceD₄₂₈sll`	`hntceD₄₂₂sll`
Block B	`xxKxaxdey`	`drK₂₈₄wayvr`	`dkK₂₉₀fiaihi`	`dnK₃₀₆tafdfv`	`dsK₄₁₂vamdey`	`dsK₄₀₂ramdey`	`dsK₃₉₆ramdey`
Block C	`frSKeisKx`	`leS₂₄₇K₂₄₈kisK₂₅₂t`	`nkS₂₅₆K₂₅₇eftK₂₆₁s`	`keS₂₇₃K₂₇₄vlsK₂₇₈d`	`frS₃₆₈K₃₆₉eisK₃₇₃s`	`frS₃₆₀K₃₆₁eisK₃₆₅s`	`frS₃₅₄K₃₅₅eisK₃₅₉s`
Block D	`ixsyNhlGNnDg`	`tmqlN₂₂₉vgG₂₃₂N₂₃₃mD₂₃₅f`	`vaqfN₂₃₈igG₂₄₁N₂₄₂mD₂₄₄f`	`wmsyN₂₅₅ilG₂₅₈dyD₂₆₁g`	`iasyN₃₅₀hlG₃₅₃N₃₅₄nD3₅₆g`	`ivsyN₃₄₂hlG₃₄₅N₃₄₆nD₃₄₈g`	`ivsyN₃₃₆hlG₃₃₉N₃₄₀nD₃₄₂g`
Block E	`F/YIngxpxxt`	`F₁₆₈vnalpvfi`	`FI₁₈₇andpa`	`Y₁₉₆anftpspgs`	`YI₂₉₃ngspqnt`	`YI₂₈₅ngspqnt`	`FI₂₇₉ngspqnt`
Block F	`F.1: lGvL/ML/MI/VG and F.2: ltagxxA`	`-`	`-`	`-`	`lG₆₇iMLIG₇₂` `tvasvlA₈₇`	`tGlLLVG₇₁` `avgsifA₈₆`	`lG₆₃vMLVG₆₈` `ltagviA₈₃`

Mt- Mycobacterium tuberculosis, Tm- Thermotoga maritima, Af- Archaeglobus fulgidus, Sc- Saccharomyces cerevisiae and Ce- Caenorabditis elegans. OsMIPS is the plant model for comparison as there is no plant MIPS crystal structure reported till now.

^a The underlined amino acids represented in upper case have ≥90% similarity across all 172 sequences studied. Locus of the individual residue in the protein sequence is denoted as subscript. The residues represented in upper case only have 50% similarity for that position. Lower case represents ≤50% similarity.