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. 2017 Sep 26;12(9):e0185498. doi: 10.1371/journal.pone.0185498

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© 2017 Siska et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) Schematic representation of a Glucose Biosensor (GB) gene used for the genetic modification of MSCs. The gene encodes a chimeric protein consisting of a signal peptide allowing protein secretion followed by Cyan Fluorescent Protein (CFP), a glucose binding domain and Yellow Fluorescent Protein (YFP). (B) Optical and YFP fluorescence microscopy of GB/hTERT MSCs (scale bar: 100μm). (C) Flow cytometry of GB/hTERT MSCs for mesenchymal CD29, 73, 90, 105 markers. Unstained cells were used as control. (D) Staining of control or differentiated GB/hTERT cells with Alizarine Red/Oil Red O indicative of osteogenic/adipogenic differentiation, respectively (scale bar: 100μm).