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. 2017 Sep 26;12(9):e0185498. doi: 10.1371/journal.pone.0185498

Fig 3. Self-renewal potential of genetically modified MSCs.

Fig 3

(A) Immunoblots for hTERT in control, GB and GB/hTERT MSC extract using an anti-hTERT antibody. GAPDH was used as a loading control (B) Relative telomerase activity in GB and GB/hTERT MSCs at passage 20. (C) Cumulative population doublings of GB and GB/hTERT MSCs over time. GB MSCs reached replicative senescence at passage 28. (D) mRNA levels of Oct-4, Sox-2 and Nanog pluripotency markers in GB and GB/hTERT MSCs (***: p<0.001, **: p<0.01). Results in (B) and (D) are shown as mean ± SD of 3 independent experiments.