Fig. 1.

pH dependence of the proliferation parameters. The parameters cell count, WST-1 metabolism and cellular ATP content were determined after six days of cell culture. All parameters were normalized to their 100% values at pH 7.4. Each point represents the mean and standard deviation of 10 measurements. The fitting procedure is described in S1 File. The dashed lines illustrate the graphically determined values ${\mathit{pH}}_{\mathit{low}}^{100\%}$, ${\mathit{pH}}_{\mathit{high}}^{100\%}$, ${\mathit{pH}}_{\mathit{max}}^{\mathit{graph}}$ and $f_{\mathit{max}}^{\mathit{graph}}$ given in Table 2. ${\mathit{pH}}_{\mathit{low}}^{100\%}$, ${\mathit{pH}}_{\mathit{high}}^{100\%}$confine the theoretical pH range, in which the fitted curves predict increased parameter values with respect to the standard pH of 7.4.