Fig. 1.

Effects of USP2 in macrophages on insulin signaling in adipocytes. Changes in insulin-elicited Akt phosphorylation (pAkt; A, C) and insulin receptor β chain (pIRβ; B, D) in 3T3-L1 cells conditioned by USP2 knockdown HL-60 cells (KD; A, B) or Usp2a overexpression isolated mouse macrophages(C, D). 3T3-L1 cells were pretreated with culture media from USP2 KD (A, B), Usp2a overexpression (C, D), or their respective control (Ctrl) cells for 10 h. After insulin (100 nM) treatment, the 3T3-L1 cells were subjected to Western blot (A, C) and immunoprecipitation Western blot (B, D) analyses. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was also detected as reference. Values presented are the mean±SD of three experiments. *P<0.05 vs. control cell-conditioned cells.