Fig. 3.
Effect of 4-OHT on CAPs recorded from frog sciatic nerve fibers. (A) 4-OHT at a concentration of 0.1 mM irreversibly reduced CAP peak amplitudes. Top of figure: recordings of CAPs in the control, at 20 min after exposure to 4-OHT, and at 30 and 60 min after washout. Bottom of figure: average time course of changes in CAP peak amplitudes following exposure to 4-OHT for 20 min, relative to that before exposure, obtained from four sciatic nerves. (B) Concentration dependency of the CAP peak amplitude reduction produced by 4-OHT. Plot of the peak amplitudes of CAPs, relative to control, recorded from sciatic nerve fibers treated with 4-OHT at various concentrations for 20 min. Each of the data points was obtained from 3 to 4 sciatic nerves. The concentration-response curve was drawn according to the Hill equation (IC50=0.26 mM, nH=1.3).