Fig 3. Heatmap of variably present Burkholderia ubonensis genes across the MSMB0022 genome.

A presence/absence matrix was constructed across 1kb windows of the MSMB0022 reference genome for each of the 264 taxa. Green = 100% mapped reads; red = 0% mapped reads. Taxa are in rows and the 1kb windows are in columns. Only regions containing <80% window coverage for at least one strain are shown, representing 2.78Mbp of the MSMB0022 B. ubonensis genome. The absence of pC3 in 4% of strains demonstrates that this megaplasmid can occasionally segregate, a finding consistent with pC3 in other Bcc species [15].