Fig 3. PK-resistant PrP detected in the brains of inoculated animals.

Representative Western blots detecting new PrP^Res in brains from ScBH(WT or K₄N)^P1 hamsters and Tg7 mice using the C-terminal R20 antiserum. Red brackets highlight the differences between the 263K scrapie brain PrP^Res and PrP^Res in the brains of the RT-QuIC product inoculated animals. Panels were cut and aligned from larger blots that all contained pertinent positive and negative controls to emphasize relevant lanes. “X” indicates a blank lane.