Fig. 7.

Location of putative transcription factor binding motifs in the pig SLC13A1 5’-flanking region. (A) Nucleotide sequence of the predicted SLC13A1 5’-flanking region (from −1000 to +50) is shown. Position +1 (arrow) denotes the putative transcription initiation site. The translation initiation ATG codon is double underlined. Boldface letters indicate the 5’-region of exon 1. A vertebrate conserved TATA box as well as putative transcription factor binding motifs are boxed, with core sequences underlined. Potential binding motifs were identified using MatInspector [23] with parameters of core >0.9 and matrix >0.8 similarities. GATA1, GATA binding factor 1; Oct-1, octamer factor 1; HNF4, hepatic nuclear factor 4; GMEB2, glucocorticoid modulatory element binding protein 2; and GATA3, GATA binding factor 3. (B) Relative locations of each transcription-factor binding site were compared to those previously reported in the 5’-flanking region of human SLC13A1 and mouse Slc13a1[24], [25].