Fig. 1.

An IgE-producing hybridoma line, IGEL b4, aggregated in the presence of specific Ags. (A, B) Three anti-TNP IgE-producing hybridoma cell lines (IGEL b4, IGEL a2 and H1 DNP-ε-26) were cultured with LPS or TNP-LPS (10 μg/ml) or the same volume of PBS (5% v/v) for 2 days. (A) Representative photomicrographs of IGEL b4 cells were shown. (B) The calculated AI values (see Supplementary Fig. S2) of each hybridoma were shown. (C, D) IGEL b4 cells were cultured with indicated Ags (1 μg/ml each) or the same volume of PBS (5% v/v) for 2 days. The AI values were shown. *p<0.05.