Fig. 3.

(A) Effects of PPNO on DNA damage induced by m-AMSA (2.0 µM) and XK469 (50 µM) in MCF-7 cells as detected by ϒH2AX signals. MCF-7 (400–500,000 cells/ml) were treated with drug in the presence or absence of 100 µM PPNO for 2 h. Cells were preincubated with PPNO in 1% FBS media before adding drugs and incubating mixtures in the complete media for an additional 2 h. The cells were washed with ice-cold PBS and collected, lysed and analyzed by the standard Western blots as described in the Section 3. (B) Quantifications of DNA damage (γ-H2AX band) shown in (A).