Fig. 6.

Luciferase assays in HepG2 cells. Reporter plasmids prepared by inserting the promoter fragments of SELENOP (−2989~+10) and HIP/PAP (−4030~+27) upstream of a firefly luciferase reporter gene in the pGL4.17 vector were transfected into HepG2 cells. After cells were exposed to 24 h of either IH or normoxia, the cells were lysed, and promoter activities of SELENOP (A) and HIP/PAP (B) were measured. All data is represented as the mean ± SE of the samples (n=3). The statistical analyses were performed using Student's t-test.