Fig. 4.

Iron administration did not affect heme, ALA, and porphyrin concentrations in medium. A: Chemiluminescent assay to detect heme concentration of medium. HPLC to detect ALA and porphyrin concentrations in medium. N = 3. B: The calculated distribution of ALA in medium after administration. Assuming that the amount of administered ALA is 100%, ALA collection rate is calculated as the percentage of ALA in each compartment 3 days after administration. Total porphyrin equivalent of ALA is the amount of total porphyrin converted to ALA.