Fig. 2.

Optimization of luciferase assay for a high throughput setting. A) Miniaturization of luciferase assay in a 384 well format. Optimal volume of reaction was obtained with the maximum luciferase activity. B) The stability of firefly luciferase activity under the reaction conditions was determined. This provided the window period over which the firefly luciferase reading could be measured without any loss of signal. C) Time taken for lysis of cells in a 384 well plate using the Passive Lysis Buffer. D) Effect of DMSO concentration on firefly luciferase activity and E) Renilla luciferase activity. F) Firefly luciferase assay was also done in Pichia pastoris wild type cells showing a similar trend and the rate of degradation as Saccharomyces cerevisiae. G) Change in firefly luciferase activity with increasing number of cells.