Fig. 2.

Increased N-WASP expression in hypoxia is dependent on HiF1α. (A) N-WASP promoter deletion constructs as indicated were generated and cloned upstream of a GFP gene. (B) HEK293T cells were co-transfected with 2 µg of N-WASP^pro-GFP constructs expressing GFP and 2 µg of HiF1α-IRES-RFP or HiF1α-STOP-IRES-RFP constructs expressing RFP. GFP and RFP fluorescence intensity was quantified as described in Materials and Methods and GFP/RFP values were plotted (N=3). ***p<0.001.