Figure 5.

Time and dose dependence of the DDR to IR in G1 cell populations. Normal prostate epithelial cells (PrEC-H), prostate cancer (DU145) cells, or chordoma (UCH-1) cells were either untreated (0 Gy) or treated with IR (8 Gy) and tested 30 minutes or 2 hours following IR for expression of p-H2AX (top left panels), pKAP1 (top middle panels), or pATM (top right panels). The DDR response was also determined following increasing doses of IR at 1 hour after treatment (bottom panels). The ratio of the positive events to the total events within the G1 DNA content gates is shown.