Figure 4.

Cell viability in rAAV-hTGF-β-transduced human osteochondral defect cultures in the presence of polymeric micelles.

Notes: Defects were prepared and incubated with rAAV/polymeric micelles and the cultures were processed after 10 days for (A) H&E staining (magnification ×10, all representative data; scale bar 200 µm) with (B) corresponding histomorphometric analyses and (C) to monitor cell viability using the LDH assay. Control conditions included the absence of copolymer or vector treatment (negative control) and application of free rAAV vector (positive control). *Statistically significant compared with negative control.

Abbreviations: hTGF, human transforming growth factor; LDH, lactate dehydrogenase; rAAV, recombinant adeno-associated virus.