Figure 6.

Hypertrophic and terminal differentiation activities in rAAV-hTGF-β-transduced human osteochondral defect cultures in the presence of polymeric micelles.

Notes: Defects were prepared and incubated with rAAV/polymeric micelles and the cultures were processed after 10 days for immunohistochemical detection of (A) type-X collagen and (C) alizarin red staining (all at magnification ×10, all representative data; scale bar 200 µm) with (B, D) corresponding histomorphometric analyses. Control conditions included the absence of copolymer or vector treatment (negative control) and application of free rAAV vector (positive control). *Statistically significant compared with negative control.

Abbreviations: hTGF, human transforming growth factor; rAAV, recombinant adeno-associated virus.