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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Neuroimage. 2017 Jun 30;158:232–241. doi: 10.1016/j.neuroimage.2017.06.060

Figure 7.

Figure 7

Pharmacogenetic manipulation of new neuron production. Experimental design (A). Suppression of SVZ neurogenesis via valganciclovir-induced ablation of GFAP-expressing neural precursor cells in GFAP-TK rats (C,E), but not WT rats. (B,C) Doublecortin-immunostaining (green) showed a reduction of DCX (+) migrating neuroblasts from the SVZ and along RMS into the OB. Naris occlusion (OCC) was performed on the WT (WT-OCC) and TK (TK-OCC) rats. Both TK (N=6) and WT (N=4) rats were subjected to naris occlusion for 3 weeks (F,H), and subsequent removal of naris plugs to allow the return of activity for another 3 weeks (G,I). MRI imaging of olfactory bulbs was performed ex vivo to obtain OB volumes. Arrows indicate experimental side. As shown in the graph (J), lack of migrating neuroblasts and addition of new neurons led to the loss of OB growth recovery after olfactory activity deprivation. ** Indicates significant difference with p < 0.05. Scale bar = 500 μm in A and B, 100 μm in C.