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. Author manuscript; available in PMC: 2018 Feb 28.
Published in final edited form as: Nat Neurosci. 2017 Aug 28;20(10):1350–1360. doi: 10.1038/nn.4630

Figure 5. Glia direct pioneer and follower-axon guidance using distinct signaling pathways.

Figure 5

(a, c) UNC-6/Netrin and MAB-20/Semaphorin regulate nerve-ring assembly. UNC-6 guides primarily pioneer SubL axons while MAB-20 specifically guides follower axons and both act from glia. (b,c) FMI-1/Flamingo/CELSR can act cooperatively from SubL commissure neurons and glia to drive nerve-ring assembly, by specifically guiding follower axons. (a–c) cDNA expression of MAB, 20, UNC-6 and FMI-1 is driven by Pmir-228 (glia), Pceh-17 or Pceh-24 (SubL neurons), or endogenous regulatory regions. Expression patterns are described in Supplementary Methods and Supplementary Tables S8, S10. Numbers inside bars: total animals scored per genotype, n=4 independent scoring experiments or number of transgenic lines in rescue experiments. Mean +/− Error bars: SEM. Numbers above bars, p values from Fisher’s exact test. ns: non significant.