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. Author manuscript; available in PMC: 2018 Jul 25.
Published in final edited form as: Biochemistry. 2017 Jul 11;56(29):3745–3753. doi: 10.1021/acs.biochem.7b00211

Figure 7.

Figure 7

Structural analysis of DNA in complex with FOXA2 and FOXO1 DBDs. (A–C) Comparison of DNA shape features between FOXA2 and FOXO1 complexes, showing (A) minor groove widths of bound and unbound DNAs, (B–C) slide and roll angles for FOXA2 and FOXO1 DBE2 DNAs. Bound DBE2 is shown in cyan (FOXA2) and magenta (FOXO1), while unbound DBE2 is shown as a dotted blue line. (D–E) Schematic diagram shows interactions between the forkhead domain and the DNA backbone as a nucleotide-residue interaction map for (D) FOXA2-DBD/DBE2 and (E) FOXO1-DBD/DBE2. Backbone contacts display slight differences between FOXA2-DBD/DBE2 and FOXO1-DBD/DBE2 complexes. Nucleotide-residue contacts (red and black lines) represent interactions that involve hydrogen bonds or other contacts, respectively. Images were obtained using DNAproDB31. Black rectangles (panels D and E) highlight the core binding element in the DBE2 DNA site.