Figure 1.

The chemotactic responses of peripheral blood PMN from patients with primary ciliary dyskinesia (PCD) are significantly lower to CXCL5 and CXCL8, but are normal to leukotriene B4 (LTB4) and complement component 5a (C5a). PMN from patients with PCD, healthy children (Ped CO) and healthy adults (Ad CO) were added to Neuro Probe chemotaxis chambers to compare their migratory capacity. The chemotactic index (CI; average number of migrated cells in response to the chemoattractant divided by the average number of spontaneously migrated cells) was used to express the chemotactic response. The chemotaxis toward (A) CXCL5 (10 ng/ml), (B) CXCL8 (10 ng/ml), (C) LTB4 (10⁻⁸ M), or (D) complement component C5a (10 ng/ml) was expressed relative to the chemotactic potency of the PMN from the reference Ad CO that was included in each Neuro Probe chemotaxis chamber on the same day (%). Each dot represents a separate blood donor and the horizontal lines indicate the median value. The number of blood donors (n) in each experimental group is indicated below the x-axis of a figure. Statistical data analysis was performed using the Mann–Whitney U test (Ad CO versus PCD: **p < 0.01, ***p < 0.001; Ped CO versus PCD: ^$$p < 0.01).