Figure 5.

Ethanol treatment increased ubiquitin signals on lipid droplets. (A and B) AML12 cells were treated with ethanol (24 hours) and CQ (last 3 hours), and then stained with anti-ubiquitin (green) and Bodipy-581/591 (red). Representative confocal images are shown (A). Boxed areas are enlarged. Lipid droplets with colocalized ubiquitin (Ub) signals are circled (A) and quantified (B). (C) AML12 cells were treated as in A, and then sequentially stained for Ub, SQSTM1 and LDs. Representative confocal images with three-color merge are shown. Paired co-localizations of ubiquitin, SQSTM1 and LDs are individually illustrated for the boxed areas of the ethanol, and ethanol/CQ-treated samples. Data represent mean ± SEM. ^** P < 0.01, ^*** P < 0.001.