Table 2.
Meyerozyma guilliermondii BI281 growth at six different combinations of carbon or nitrogen sources.
| Carbon/Nitrogen source | Time (h) | X (g L−1) | L (g L−1) | Lmax (%) | Ymax L X−1 t−1 (g/g/h) |
|---|---|---|---|---|---|
| Glucose/(NH4)2SO4 | 24 | 1.9953 ± 0.1275 | 1.5247 ± 0.2659 | 76.41 | 0.0318 |
| 48 | 2.2380 ± 0.2008 | 1.4770 ± 0.2984 | 65.99 | 0.0137 | |
| 72 | 2.4067 ± 0.2299 | 1.0580 ± 0.6633 | 43.96 | 0.0061 | |
| 96 | 2.5007 ± 0.4146 | 1.3100 ± 0.2348 | 52.39 | 0.0055 | |
| Glycerol/(NH4)2SO4 | 24 | 2.3733 ± 0.0811 | 0.6266 ± 0.0277 | 26.43 | 0.0110 |
| 38 | 3.6944 ± 0.1195 | 0.6353 ± 0.0810 | 17.41 | 0.0046 | |
| 48 | 4.2649 ± 0.2804 | 0.7688 ± 0.0539 | 18.76 | 0.0039 | |
| 72 | 5.0889 ± 0.4714 | 0.7590 ± 0.0984 | 14.15 | 0.0020 | |
| 96 | 3.5633 ± 0.3523 | 1.2362 ± 0.0387 | 34.97 | 0.0036 | |
| 120 | 5.5484 ± 1.1415 | 1.3450 ± 0.3687 | 22.10 | 0.0018 | |
| Glucose/Malt extract | 96 | 0.9747 ± 0.1897 | 0.5400* | ND | 0.0077* |
| Glucose/NH4NO3 | 96 | 0.9473 ± 0.1099 | 0.1393 ± 0.0133 | 14.71 | 0.0020 ± 0.0017 |
| Glucose/Peptone | 96 | 5.6580 ± 0.2910 | 2.6613 ± 0.1453 | 47.04 | 0.0065 ± 0.0069 |
| Glucose/Tryptone | 96 | 5.9173 ± 0.2453 | 2.8953 ± 0.2404 | 48.93 | 0.0068 ± 0.0136 |
Biomass (X), lipid production (L), lipid productivity (Ymax L/X/t) and maximum concentration of cellular lipids (Lmax) (%, according to total cell dry weight) are represented. All culture media were supplemented with 0.1% KH2PO4 and 0.05% MgCl26H2O. The culture conditions were 150 rpm, 26°C for 96 h for glucose and 120 h for glycerol.
*
Value without triplicate. For this reason, the estimation of Lmax is not shown (ND).