Table 3.
Experimental design to improve M. guilliermondii BI281 lipid content, and comparison of observed data, data predicted by the model and residues.
| Run | Time (h) | C/N ratio | Stirring (rpm) | Lipid content (mg/L) | ||
|---|---|---|---|---|---|---|
| Observed value | Predicted value | Residues | ||||
| 4 (c) | 72 | 100:1 | 150 | 100.4719 | 126.4801 | −26.0082 |
| 2 | 72 | 100:1 | 276 | 184.5952 | 162.8444 | 21.7508 |
| 12 | 96 | 50:1 | 225 | 57.2506 | 57.1579 | 0.0927 |
| 14 | 96 | 150:1 | 225 | 192.5581 | 185.1536 | 7.4045 |
| 5 (c) | 72 | 100:1 | 150 | 198.7951 | 126.4801 | 72.3150 |
| 6 (c) | 72 | 100:1 | 150 | 106.1470 | 126.4801 | −20.3331 |
| 15 | 72 | 100:1 | 24 | 142.7192 | 90.1158 | 52.6034 |
| 8 | 72 | 16:1 | 150 | 16.8804 | 5.4506 | 11.4298 |
| 10 | 48 | 50:1 | 75 | 55.7712 | 113.4219 | −57.6507 |
| 1 | 48 | 150:1 | 75 | 111.6914 | 119.8489 | −8.1576 |
| 13 | 48 | 150:1 | 225 | 192.5581 | 190.2689 | 2.2892 |
| 16 | 32 | 100:1 | 150 | 110.2632 | 134.2331 | −23.9699 |
| 9 | 112 | 100:1 | 150 | 14.0873 | 69.6156 | −55.5284 |
| 11 | 96 | 50:1 | 75 | 72.8236 | 40.9963 | 31.8273 |
| 3 | 96 | 150:1 | 75 | 10.1330 | 18.1979 | −8.0648 |