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. 2017 Sep 22;8:1173. doi: 10.3389/fimmu.2017.01173

Figure 3.

Figure 3

Reconstitution of miR-155 in natural killer (NK) cells from immune-activation (IA) patients led to decreases in suppressor of cytokine signaling 1 (SOCS1) expression and increases in cytokine production. Purity of NK cells from IA patients was transfection with miR-control or miR-155-mimic for 72 h. (A–C) After stimulated with IL-12 (10 ng/ml), IL-15 (10 ng/ml), and IL-18 (100 ng/ml) for 24 h, IFN-γ and TNF-α secretion of isolated NK cells were detected by flow cytometric analysis. A representative pattern (A) and statistics analysis of IFN-γ (B) and TNF-α (C) secretion of transfected NK cells determined by flow cytometric analysis. (D) After 72 h transfection, SOCS1 mRNA expression in isolated NK cells was determined by real-time RT-PCR. Control, miR-control; miR-155, miR-155 mimic.