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. 2017 Sep 26;7:12297. doi: 10.1038/s41598-017-12069-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Decreased viability of RTT fibroblasts in starvation medium. (a) Time-dependent viability of RTT and healthy fibroblasts cultivated over 24 h in the starvation medium. Results are reported as percentage of living cells vs the number of cells at time 0 of the assay. Results presented are the means ± S.E. of five independent experiments performed in triplicate. *Significantly different from healthy cells at time 0; **significantly different from either RTT cells time 0 and from the viability of healthy cells at the corresponding time point (*p < 0.01, **p < 0.05, oneway ANOVA, followed by Tukey’s test, n = 15). (b) Western blotting analysis of the p25 fragment of PARP (Poly (ADP-ribose) polymerase (PARP), a family of proteins involved in a number of cellular processes concerning mainly DNA repair and programmed cell death) in RTT and healthy fibroblasts grown under starving condition for 2 and 4 h. Beta-tubulin was used as internal control. A representative immunoblot of three independent experiments is reported. Results presented are the means ± S.E. of three independent experiments performed in triplicate. *Significantly different from control (either healthy and RTT cells at time 0) (*p < 0.001, one-way ANOVA, followed by Tukey’s test, n = 9).