Fig. 8.

Cartoon model of how mechano-inhibition of formin Cdc12 is critical for facilitating proper ring assembly from cytokinesis nodes. By slowing down in vivo F-actin polymerization rates to a level where Myo2 pulling net velocity is sufficiently larger (Fig. 7i), mechano-inhibition of formin Cdc12 facilitates effective node coalescence (Fig. 7h). In addition, mechano-inhibition of formin Cdc12 prevents excessive F-actin generation, which would impede broad band formation once neighboring nodes are inter-linked upon capture (Fig. 7a–f)