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. 2017 Sep 26;7:12341. doi: 10.1038/s41598-017-12563-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical staining of TF expression in glioma specimens performed using anti-TF 1849 mAb. (a,b) The left panels show HE staining and the right panels show TF staining with weak intensity (a) and strong intensity (b) in GBM. Scale bar, 200 µm. (c) High-power field of (b). HE staining (left panel) shows morphological findings of necrosis surrounded by pseudopalisading cells in GBM. TF was highly expressed in necrosis and pseudopalisading cells (right panel). Scale bar, 100 µm. (d) The level of TF was classified into four degrees: (−), negative; (+), <50% positive tumour cells; (++), ≥50% positive tumour cells with weak intensity; and (+++), ≥50% positive tumour cells with strong intensity. The percentages of the four categories in grade 2, 3 and 4 gliomas are shown. Each group consisted of 25 cases.