Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 26;7:12341. doi: 10.1038/s41598-017-12563-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The affinity and specificity of fluorescent- and radiolabelled antibodies for TF expression in glioma cell lines. (a) The affinity of Alexa Fluor 647-labelled anti-TF 1849 IgG and control IgG for TF expressed on U87MG/Luc (upper) and U87MG/Luc/TF cells (lower) was evaluated using flow cytometry. The relative intensity of Alexa Fluor 647-labelled anti-TF 1849 IgG normalized to the negative control in U87MG/Luc and U87MG/Luc/TF was 4.25 and 158.06, respectively. The relative intensity of Alexa Fluor 647-labelled control IgG in U87MG/Luc and U87MG/Luc/TF was 1.23 and 1.28, respectively. (b) To evaluate the affinity and specificity of ¹¹¹In-labelled anti-TF 1849 IgG and control IgG, we examined the capacity of the radiolabelled antibodies to bind to serially diluted U87MG/Luc and U87MG/Luc/TF cells. The radioactivity derived from ¹¹¹In-labelled anti-TF 1849 IgG bound to U87MG/Luc/TF cells increased in a cell number-dependent manner. In contrast, the increased radioactivity in U87MG/Luc cells was considerably lower compared to in U87MG/Luc/TF cells. However, ¹¹¹In-labelled control IgG showed negligible binding to both U87MG/Luc and U87MG/Luc/TF cells.