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. 2017 Sep 26;7:12329. doi: 10.1038/s41598-017-12190-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Average force-displacement measurement (black line) using force-sensing microprobes, indenting the animal in a region close to the vulva either posteriorly or anteriorly (n = 9 animals). Computer simulation using an absolute E _o and E _i value of 140/70 kPa (red line) and 300/60 kPa (blue line) respectively to find a matching force-displacement curve to the mean of experimentally obtained measurements. Blue dashed line is 0–10 µm and blue solid line is 10–14 µm. Values are the averaged data ± s.e.m.