Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 26;8:702. doi: 10.1038/s41467-017-00768-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — CD4-skewed development by impaired Thpok regulation from hypomorphic Bcl11b ^m/m progenitors. a Expression of truncated Bcl11b protein in the neonatal thymus from the hypomorphic Bcl11b ^m allele detected by an antibody recognizing the N- (left), but not the C- (right), terminus of Bcl11b. One representative of two experiments. b Summary of ChIP assay for binding of Wt (white bars) and hypomorphic (black bars) Bcl11b to indicated regions in total neonatal thymocytes. c−e Flow cytometry analyzing the expression of CD4, CD8, and Thpok-GFP during T cell development in Rag1-deficient hosts c and irradiated MHC-II deficient hosts e reconstituted with fetal liver cells with the indicated genotypes. Data are representative of at least two independent experiments. Numbers in dot plots indicate the percentage of cells in each quadrant. Histograms showing CD40L expression 2 days after in vitro TCR stimulation of peripheral CD4⁺, CD8⁺ and CD4⁻CD8⁻ DN cells differentiated in Rag1-deficient mice from Bcl11b ^+/+and Bcl11b ^m/m fetal liver cells d. Data are representative of two independent experiments