Fig. 3.

Sth-independent Thpok repression by Bcl11b in pre-selection DP thymocytes. a Quantitative RT-PCR showing amount of promoter-specific Thpok transcripts in splenic CD4⁺ T cells and splenic B220⁺ B lymphocytes. Summary of three measurements. b Quantitative RT-PCR for Thpok-gfp expression in CD4⁺ T cells from mutant reporter alleles, Thpok ^gfp:ΔTE and Thpok ^gfp:ΔPE, lacking the thymic enhancer (TE) or proximal enhancer (PE), respectively. Levels of P1- and P2 promoter-specific transcripts relative to those from the control Thpok ^gfp allele harboring intact enhancers is shown. One representative of two independent experiments. c Promoter-specific Thpok transcripts in Bcl11b ^m/m pre-selection DP thymocytes and CD4⁺ T cells relative to those in control CD4⁺ T cells. Summary of three measurements. d Histograms showing Thpok-GFP expression from the Thpok ^gfp:ΔTESPE allele in the indicated cell subsets differentiated in Rag1-deficient recipients from control (dotted line) and Bcl11b ^m/m (solid line) fetal liver cells. Expression pattern of Thpok ^gfp is shown as reference (shaded). Schematic structure of the Thpok ^gfp:ΔTESPE allele is included (upper panel). One representative of two independent experiments. e ATAC-seq tracks at the Thpok locus in CD24^hiTCRβ^loCD4⁺CD8⁺ pre-selection thymocytes of 6-week-old Bcl11b ^+/+ mice, newborn Bcl11b ^+/fl :Lck-cre, newborn Bcl11b ^HM/fl :Lck-cre and newborn Bcl11b ^HM/HM :Lck-cre mice. ATAC-seq results in immature B cells (GSM2123187) is shown as reference. DP thymocyte- and B lymphocyte-specific peaks are marked with blue and red arrowheads, respectively. Position of the P1 promoter is indicated with black arrowhead