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. 2017 Sep 26;8:702. doi: 10.1038/s41467-017-00768-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Lineage scrambling resulting from inactivation of Bcl11b at the DP stage due to sporadic Thpok and Runx3 expression. a Flow cytometry analyzing the expression of CD4, CD8, Thpok-GFP, and Runx3-tdTomato by various thymocyte subsets in mice with the indicated genotypes. The histogram in the middle showing Thpok-GFP expression in CD4⁺CD8⁻ mature thymocytes from control (open) and Bcl11b-deficient (shaded) cells. b Graph showing summary of percentage of each subset in mature (CD24⁻TCRβ^hi) thymocytes population of mice with indicated genotype. c Flow cytometry analyzing the expression of CD4, CD8, Thpok-GFP and Runx3-tdTomato in lymph node T cells of mice with the indicated genotypes. One representative of at least independent mice. d Flow cytometry analyzing the expression of CD4, CD8, Thpok-GFP and Runx3-tdTomato in pre-selection DP thymocytes and lymph node T cells differentiated in Rag1-KO hosts from Bcl11b ^+/+ and Bcl11b ^m/m fetal livers harboring Thpok ^gfp and Runx3 ^tdTomato reporter alleles. One representative of two independent experiments. e, f Representative flow cytometry e analyzing differentiation of MHC-I and MHC-II selected cells in MHC-II and MHC-I deficient (MHC-II⁰ and MHC-I⁰) backgrounds, respectively, along with a graph f showing a statistical summary of the percentages and absolute numbers in each cell subset in CD24⁻TCRβ^hi mature thymocytes population of control (white circles) and Bcl11b ^fl/fl :Cd4-Cre (filled circles) mice. Partial re-directed differentiation occurred in both MHC-I and -II selected cells. *P < 0.05, **P < 0.01, and ***P < 0.001(unpaired t-test)