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. 2017 Sep 26;7:12336. doi: 10.1038/s41598-017-12101-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of influenza virus in cytopathic effect inhibition assays of novel inhibitors. (A) Infection of MDCK cultured cells with influenza viruses was conducted in the presence of different inhibitor concentrations. The cells are stained with crystal violet after incubation. The presence of the blue color indicates living cells without the viral infections. Absence of blue color indicates the infecting virus causes lysis of the host cell. Virus control (VC) and cell control (CC) are listed as shown. Numbers above each well represents concentration of inhibitor used in µM. (B) The mean IC₅₀ values of the inhibitors against H1N1 virus and the stare listed as shown. Experiments performed in triplicates with the standard of deviation for each mean listed in table.