Figure 2.

Optimization of the conditions for immobilizing the DNAzyme sensor and results of long-term stability tests. (A) Fluorescent image after the cleavage reaction of the DNAzyme 24 h after printing. The DNAzyme probe was printed with 4 different conditions: (1) with reaction buffer (RB) alone, (2) with RB including trehalose (TH), (3) with RB including pullulan (PL), and (4) RB including TH and PL. EC stands for E. coli. (B) Denaturing gel image of the reaction mixtures of the experiments in A (Full gel image is provided in the ESI, Fig. S2A, with the dashed line box delineating the section used to produce Fig. 2B.) (C) Fluorescent image after the cleavage reaction of the DNAzyme after 7 days of storage using the same printed components described in A. (D) Denaturing gel image of the reaction mixtures of the experiments in C (Full gel image is provided in the ESI, Fig. S2B with dashed line box delineating the section used to produce Fig. 2D.) (E) Stability test of the DNAzyme after printing on paper using the optimized conditions. The image is a representative denaturing gel image of the cleavage reaction on paper (individual gel images were obtained at each storage time; the original gel images are provided in the ESI Fig. S2B–D. The sections used to construct composite Fig. 2E are indicated with dashed boxes).