Fig. 3.

(a): Effect of various concentrations of carbofuran (0.025, 0.25, 0.50, 1.00, 2.50, 5.00 μM) on lipid peroxidation in the supernatant of homogenate (10%, w/v) prepared in 0.25 M sucrose solution from rat liver slices in vitro. Control: control group had not treated with any treatment. Level of MDA was determined as described in Materials and Methods. The unit of level of MDA is mg⁻¹ Protein. The values were expressed as Mean ± SD. The sign (*), (**), and (***) indicates values significantly increased from control at (P < 0.05). Carbofuran at doses of 0.5, 1.0, 2.5 and 5.0 μM has been observed to be significantly increased. (b): Effect of various concentrations of carbofuran (0.025, 0.25, 0.50, 1.00, 2.50, 5.00 μM) on lipid peroxidation in the supernatant of incubation medium of rat liver slices in vitro. Control: control group had not treated with any treatment. Level of MDA was determined as described in Materials and Methods. The unit of level of MDA is mg⁻¹ Protein. The values were expressed as Mean ± SD. The sign (*), (**), and (***) indicates values significantly increased from control at (P < 0.05). Carbofuran at doses of 0.5, 1.0, 2.5 and 5.0 μM has been observed to be significantly increased. (c): Time dependent alterations in MDA levels in the supernatant of homogenate (10%, w/v) prepared in 0.25 M sucrose solution from rat liver slices after incubation with 2.5 μ moles of carbofuran in vitro. Control: control group had not treated with any treatment. Level of MDA was determined as described in Materials and Methods. The unit of level of MDA is mg⁻¹ Protein. The values were expressed as Mean ± SD. The sign (***) indicates values significantly increased from control at (P < 0.05). (d): Time dependent alterations in MDA levels in the supernatant of homogenate (10%, w/v) in 0.25 M sucrose solution of the supernatant from the incubation medium of rat liver slices after incubation with 2.5 μ moles of carbofuran in vitro. Control: control group had not treated with any treatment. Level of MDA was determined as described in Materials and Methods. The unit of level of MDA is mg⁻¹ Protein. The values were expressed as Mean ± SD. The sign (***) indicates values significantly increased from control at (P < 0.05). (e): Effect of vitamin C on carbofuran induced changes on the level of lipid peroxidation from the supernatant of homogenate (10%, w/v) prepared in 0.25 M sucrose solution of rat liver slices in vitro. Control: control group had not treated with any treatment. The experimental groups of liver slices were treated with different conditions as described in materials and methods. The level of MDA was determined as described in Materials and Methods. The unit of level of MDA is mg⁻¹ Protein. The values were expressed as Mean ± SD. The sign (*) indicates values significantly increased from control at (P < 0.05). (f): Effect of vitamin C on carbofuran induced changes on the levels of lipid peroxidation in the supernatant of the incubation medium in vitro. Control: control group had not treated with any treatment. The experimental groups of liver slices were treated with different conditions as described in materials and methods. The level of MDA was determined as described in Materials and Methods. The unit of level of MDA is mg⁻¹ Protein. The values were expressed as Mean ± SD. The sign (*) indicates values significantly increased from control at (P < 0.05).