FIG 1.

Identification of PTMs on 14-3-3ζ and SOD1. (A) Brain, liver, and whole-embryo mouse tissues were homogenized and digested with trypsin. Peptides were subjected to affinity purification by the indicated antibody resin. Peptides were eluted and analyzed by LC–MS-MS. Proteomics data were analyzed with Scaffold software. IP, immunoprecipitation. (B) Crystal structures of human 14-3-3ζ (PDB accession no. 4IHL) and mouse SOD1 (PDB accession no. 3GTT) with PTMs identified in the proteomics data.