FIG 6.

Thr⁵⁰⁷ phosphorylation regulates δKD-dependent responses in a cellular context. Untransfected HEK293 cell cultures (−) and HEK293 cells that heterologously overexpress similar levels of WT-δKD or δKD-T507A were subjected to immunoblot analysis to screen for overall PKC substrate phosphorylation at the pS-PKC substrate (R/L-X-pS-ϕ-R/L) or TXR motifs (left) or the phosphorylation of various mitogen-activated protein kinases (ERK, SAPK/JNK, and p38-MAPK) or AKT (right). Similar results were obtained in three separate experiments.