FIG 7 .

Binding and virus neutralization of type-specific neutralizing MAbs A12A3 and 28F10 can be redirected to the chimeric particles of HPV33 and HPV18, respectively. (A) Sequence alignments of L1 proteins of HPV58, -33, -18, and -59. The consensus was determined based on the alignment of L1 sequences from 36 different HPV serotypes (also see Fig. S8 in the supplemental material). The six variant regions (BC, DE, EF, FG, HI, and the C-terminal arm) are labeled. The residues involved in the antibody binding are marked by solid triangles for HPV58 and open triangles for HPV59. The different residues between HPV58 and HPV33 among the epitope residues against A12A3 are highlighted in red, and those between HPV18 and HPV59 against 28F10 are highlighted in blue. (B and C) ELISA for evaluation of binding of chimeric VLPs of HPV33 and HPV18 against MAbs A12A3 (B) and 28F10 (C). (D and E) Neutralization assay of MAbs A12A3 and 28F10 to WT and mutant PsVs of HPV33 (D) and HPV18 (E).