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. 2017 Sep 22;8:1816. doi: 10.3389/fmicb.2017.01816

Table 1.

Plasmids used in this work.

Plasmids Plasmid characteristics Reference
pC194 Natural rolling circle replicating (RCR) plasmid, 2.9-kb Horinouchi and Weisblum, 1982; Alonso and Trautner, 1985
pHP13 RCR plasmid derivative of pTA1060, 4.9-kb Haima et al., 1987
pBT163 (pHP13-pac) pHP13 derivative containing SPP1 DNA including pac (2675 bp cloned, coordinates 43778–44010 and 1–2439) Chai et al., 1992
pBT271 (pHP13-oriL) pHP13 derivative containing SPP1 DNA including oriL (2975 bp, coordinates 33875–36850) Chai et al., 1993
pBT400 (pHP13-800) pHP13 derivative containing SPP1 DNA (864 bp, coordinates 3225–4089) This work
pUB110 Natural RCR plasmid, 4.5-kb Leonhardt, 1990
pUB110-cop1 pUB110 derivative, lower copy number Leonhardt, 1990
pBG55 (pUB110-3600) pUB110 derivative containing SPP1 DNA (3639 bp, coordinates 23117–26756) Deichelbohrer et al., 1985
pBT233 Theta replicating (TR) plasmid, 9-kb Ceglowski et al., 1993a
pBT233N pBT233 derivative containing the 1304 bp neomycin resistance gene (N) from pUB110 This work
pBT233N-400 pBT233N derivative containing SPP1 DNA (414 bp, coordinates 32562–32976) This work
pBT233N-1300 pBT233N derivative containing SPP1 DNA (1340 bp, coordinates 25051–26391) This work
pBT233N-oriL pBT233N derivative containing SPP1 oriL DNA (350 bp, coordinates 35801–36151 This work
pBT233N-pac pBT233N derivative containing SPP1 pac DNA (412 bp, coordinates 43689–44010 and 1–70) This work
pNDH33 TR plasmid derivative of pBS72, 8.1-kb Titok et al., 2003
pNDH33-1300 pNDH33 derivative containing SPP1 DNA (1340 bp, coordinates 25051–26391) This work
pNDH33-pac pNDH33 derivative containing SPP1 pac DNA (412 bp, coordinates 43689–44010 and 1–70) This work