Table 3.

Viral replication and recombination proteins are not involved in the establishment of transduced plasmids.

Donor straina and plasmid	Recipient strain	Phage	Transduction Frequencyb	SDc	TFM/TFwt
BG295 pBG55	BG214	SPP1 wt	4.3 × 10-3	±2.0 × 10-3	1.00d
BG295 pBG55	BG214	34.1-	3.4 × 10-3	±1.5 × 10-3	7.9 × 10-1
BG295 pBG55	BG214	35-	2.1 × 10-3	±1.1 × 10-3	4.9 × 10-1
BG295 pBG55	BG214	36-	3.4 × 10-3	±1.8 × 10-3	7.9 × 10-1
BG214 pBG55e	BG214	38-	4.3 × 10-3	±2.1 × 10-3	1.0 × 100
BG295 pBG55	BG214	39-	2.9 × 10-3	±2.0 × 10-3	6.7 × 10-1

^aBG214 is the wild type strain and BG295 is the isogenic sup3 strain. ^bThe transduction frequency (Neo^R/CFU) is the average of at least three independent experiments. ^cSD: standard deviation. ^dThe frequency of pBG55 plasmid transduction with the phage mutants (TF^M) with respect to the wt phage (TF^wt) is presented. ^eThe 38^- mutant is a thermosensitive phage (tsB3), therefore the infection was done at permissive temperature (30°C) and the transduction at non-permissive temperature (50°C) to have the mutation only in the recipient strain.