Table 4.
Transduction frequency of theta and rolling circle replicating plasmids without sequence homology with SPP1.
| Plasmid | AbR marker | Replication mechanisma | Copy numberb | ssDNA productionc | pseudo-pac sited | Transduction Frequencye | CI0.95f |
|---|---|---|---|---|---|---|---|
| pUB110 | Nm | RCR | 50 | + | – | 2.1 × 10-5 | ± 1.3 × 10-5 |
| pUB110-cop1 | Nm | RCR | 15 | + | – | 4.3 × 10-6 | ±2.2 × 10-6 |
| pC194 | Cm | RCR | 15 | +++ | – | 5.2 × 10-5 | ±4.7 × 10-5 |
| pHP13 | Cm | RCR | 5 | +++ | – | 5.9 × 10-6 | ± 2.9 × 10-6 |
| pBT233N | Nm | TR | 8 | – | – | 8.2 × 10-8 | ±7.1 × 10-8 |
| pNDH33 | Cm | TR | 6 | – | 1 | 6.4 × 10-7 | ±3.2 × 10-7 |
aRCR, rolling circle replication; TR, theta replication. bPlasmid copy numbers were reported in the literature and are presented here for comparison. cssDNA production was reported in the literature and is presented here for comparison. dThe pac motif (5′-CTATTGCGG⇓C-3′) is absent in all of the plasmids. Here the presence of a shorter motif that we call pseudo-pac site 5′-TTGCGG⇓CW-3′ is indicated. eThe transduction frequency (transductans/CFU) is the mean of at least five independent experiments. fCI, confidence interval.