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. 2017 Sep 22;8:1649. doi: 10.3389/fpls.2017.01649

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Copyright © 2017 Han, Jin, Yao, Kong, Huang, Tao, Li, Wang and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of GEG and GhMIF by qRT-PCR. Detection of the expression level of (A) GEG and (B) GhMIF in S5 ray petals under GA (gibberellic acid, 10 μM), ABA (abscisic acid, 50 μM), PAC (paclobutrazol, 10 μM), FLU (fluridone, 0.1 μM) and mock treatments at different time points (0, 4, 8, 12, 16, and 24 h). (C) Relative expression level of GEG and GhMIF during petal developmental phases (S1–S6, “S” represents “stage”) and (D) in different tissues and floral organs of the ray petals. GhACTIN (AJ763915) was used as an internal control. The experiment was repeated three times, the lowest expression in any tissue was set to 1.0, and the expression value indicates mean ± SD. The significant differences were evaluated by Tukey’ HSD test: ^∗p < 0.05, ^∗∗p < 0.01.