Table 1.
Primers for VDR SNPs and PCR-RFLP conditions.
SNP | Primer Sequence | Restriction Enzyme | PCR/RFLP Products (bp) |
---|---|---|---|
Fok-I | Fok1R: 5-ATGGAAACACCTTGCTTCTTCTCCCTC-3 Fok11F: 5-AGCTGGCCCTGGCACTGACTCtGGCTCT-3 |
FastDigest Fok-I | ff: 198, 69 FF: 267 Ff: 267, 198, 69 PCR product: 267 |
Bsm-I | ABsm1F: 5-CGGGGAGTATGAAGGACAAA-3 ABsm1R: 5-CCATCTCTCAGGCTCCAAAG-3 |
FastDigest Bsm-I | bb: 243, 105 BB: 348 Bb: 348, 243, 105 PCR product: 348 |
Taq-I | ATaq1F: 5-GGATCCTAAATGCACGGAGA-3 ATaq1R: 5-AGGAAAGGGGTTAGGTTGGA-3 |
FastDigest Taq-I | tt: 225, 200, 205 TT: 425, 205 Tt: 425, 225, 200, 205 PCR product: 630 |
Apa-I | ATaq1F: 5-GGATCCTAAATGCACGGAGA-3 ATaq1R: 5-AGGAAAGGGGTTAGGTTGGA-3 |
FastDigest Apa-I | aa: 484, 146 AA: 630 Aa: 630, 484, 146 PCR product: 630 |